The new coronavirus known as SARS-Cov-2 (severe acute respiratory syndrome -coronavirus 2) was first reported in December 2019 and rapidly became a public health emergency. The COVID-19 pandemic is now affecting sensitive regions with fragile health care systems, such as South America and Africa. Caregivers, in the front line of Covid19 patient management, may accidentally become infected and a source of infection during the incubation phase or in case of asymptomatic infection. The objectives of this project are thus i) to assess SARS-Cov-2 spread over the hospital departments of Bamako by carrying out a systematic molecular screening of patients and caregivers, ii) to evaluate the feasibility of Point-Of-Care molecular assays in Mali and iii) to estimate the immunity acquired from SARS-Cov-2 among health workers through serological testing, allowing also the assessment of asymptomatic caregiver rate and absence of re-infection among the immunized caregivers. Finally, iv) variability of the virus over time and spread of different variants around the world will be studied by sequencing the viral genome.
Diagnostic Test: SARS-CoV-2 screening by molecular biology
SARS-CoV-2 RT-PCR in nasopharyngeal swab targeting 2 regions of the viral genome
Diagnostic Test: Serological screening
Serological screening of caregivers at D0, M1, M2, M3 (anti-nucleocapsid antibodies).
Inclusion Criteria:
- Free and informed consent accepted in writing
- Patient: patient hospitalized in one of the four hospitals of Bamako, with clinical
signs of infection of the upper or lower respiratory tracts (sore throat,
cough/sputum, nasal congestion and rhinorrhea, odynophagia, thoracic oppression,
dyspnea, desaturation) with fever or feeling of fever or any other signs of SARS-Cov-2
infection (that is to say the following clinical manifestations, of sudden onset:
unexplained asthenia, unexplained myalgia, headache without a known migraine disease,
anosmia or hyposmia without associated rhinitis, dysgueusia, diarrhea, heart rhythm
disorders, acute myocardial injury, severe thromboembolic event) or who have been in
close contact with a SARS-CoV-2 infected person without effective protective measures
(FFP2/surgical masks or physical separation) and defined as follows: having shared the
same place of life as the confirmed case, for example: family, same room or having a
direct contact in face to face with less than 1 meter from the confirmed case during a
talk; intimate friends; class or office neighbours; adjacent to the index case in a
plane or a train; or having provided or received hygiene or care acts from a confirmed
case.
- Caregivers: caregivers of one of the four hospitals of Bamako
Exclusion Criteria:
- Persons subject to legal protection or not able to give a free and informed consent.
- Caregivers: caregivers not able to follow the project schedule
Hopital du Point-G
Bamako, Mali
Hopital Gabriel Toure
Bamako, Mali
Hôpital dermatologique de Bamako
Bamako, Mali
Hôpital du Mali
Bamako, Mali
Almoustapha Issiaka MAIGA
+223 76229920
amaiga@icermali.org