Rationale: The investigators hypothesize that genetics and the nasal epithelial response to SARS-CoV2 are critical determinants of the immune response to viral infection, and predict clinical outcome in COVID-19 patients. Objective: The main objective is to assess whether genetic background and/or the nasal epithelial gene expression in response to SARS-CoV2 is different in patients with mild, severe or very severe disease. The secondary goal of this study is to investigate a) the role of the ACE2-AngII system during SARS-CoV2 in relation to outcome b) the long-term consequences of mild, severe, and very severe COVID-19 infection c) the association between mild, severe and very severe COVID-19 with clinical & molecular markers of disease progression d) whether the faeces microbiome, virome or metabolomics profile predicts clinical outcome in COVID-19 patients and e) to investigate whether pre-existing antibodies towards other coronaviruses play a role in severe disease development. Study design: Prospective open label clinical observational study. In this study samples will be collected from 150 COVI-19 patients ( 50 mild (group 1), 50 severe (group 2) and 50 very severe (group 3) ). Blood, nasal brushes and stool will be collected for all groups at hospital admission and 3 months after recovery, and for groups 2 & 3 at day 3, at day 14, and before detubation Study population: A total of 150 patients diagnosed with COVID-19 will be included. The investigators aim for 50 patients per group, divided over 3 groups: Group 1 Patients with mild disease who tested positively for SARS-CoV2 infection, but only experience mild symptoms and do not need hospitalization. Group 2 Patients with severe disease admitted to hospital, without the need to be admitted to the intensive care. Group 3 Patients with very severe disease admitted to intensive care, who require mechanical ventilation. Main study parameters/endpoints: The primary endpoint of this study is the identification of genes, pathways and cell populations that associate with clinical outcome and disease progression in mild, severe and very severe COVID-19 patients.
Rationale:
The currently ongoing world-crippling pandemic with the new SARS-CoV2 virus shows the
desperate and urgent need for a better understanding of the biological pathways activated by
the virus inducing Coronavirus disease 2019 (COVID-19) in infected patients. A subset of
COVID-19 patients develop very severe respiratory symptoms requiring hospital admission with
or without the need for mechanical ventilation, whereas others experience mild flu-like
symptoms. The biological mechanisms underlying this striking difference in clinical outcome
and the long term consequences are unknown, yet a genetic factor seems likely. SARS-CoV2
enters cells via the ACE2 receptor and the activating protease TMPRSS2 which have recently
been shown to be strongly enriched in nasal epithelium. The investigators hypothesize that
genetics and the nasal epithelial response to SARS-CoV2 are critical determinants of the
immune response to viral infection, and predict clinical outcome in COVID-19 patients.
Objective:
The main objective is to assess whether genetic background and/or the nasal epithelial gene
expression in response to SARS-CoV2 is different in patients with mild, severe or very severe
disease. The secondary goal of this study is to investigate a) the role of the ACE2-AngII
system during SARS-CoV2 in relation to outcome b) the long-term consequences of mild, severe,
and very severe COVID-19 infection c) the association between mild, severe and very severe
COVID-19 with clinical & molecular markers of disease progression d) whether the faeces
microbiome, virome or metabolomics profile predicts clinical outcome in COVID-19 patients and
e) to investigate whether pre-existing antibodies towards other coronaviruses play a role in
severe disease development.
Study design:
Prospective open label clinical observational study. In this study samples will be collected
from 150 COVI-19 patients ( 50 mild (group 1), 50 severe (group 2) and 50 very severe (group
3) ). Blood, nasal brushes and stool will be collected for all groups at hospital admission
and 3 months after recovery, and for groups 2 & 3 at day 3, at day 14, and before detubation.
Study subjects will be retrospectively allocated to the groups.
Study population:
A total of 150 patients diagnosed with COVID-19 will be included. The investigators aim for
50 patients per group, divided over 3 groups:
Group 1 Patients with mild disease who tested positively for SARS-CoV2 infection, but only
experience mild symptoms and do not need hospitalization.
Group 2 Patients with severe disease admitted to hospital, without the need to be admitted to
the intensive care.
Group 3 Patients with very severe disease admitted to intensive care, who require mechanical
ventilation.
Main study parameters/endpoints:
The primary endpoint of this study is the identification of genes, pathways and cell
populations that associate with clinical outcome and disease progression in mild, severe and
very severe COVID-19 patients.
Inclusion Criteria:
1. Patients have to be tested (PCR) positive for SARS-CoV2 infection.
2. Provided written informed consent
Exclusion Criteria:
1. No comprehension of Dutch or English language