The aim of this effort is to study host-pathogen interaction in Egyptian patients infected with COVID-19. The investigators will perform genome-wide miRNA and transcriptome screens in the infected patients along with healthy ones for comparison. All types of cytokines play pivotal roles in immunity, including the responses to different viral infections. Therefore, The investigators will study the cytokines profile in response to that infection. By comparing miRNA and transcriptome screens along with cytokines profiles, an important molecule might be identified that could play role in the inhibition of the COVID-19 outbreak. In addition, this information will help us gaining awareness of the immune process and knowing about the genes involved in the immune response against COVID-19 with an emphasis on the expression of cytokines.
Coronaviruses are a group of viruses that cause diseases in mammals and birds. In humans,
coronaviruses cause respiratory tract infections that can be mild, such as some cases of the
common cold, and others that can be lethal, such as severe acute respiratory syndrome (SARS),
(middle east respiratory syndrome) MERS, and COVID-19. They contain a positive-sense,
single-stranded RNA genome. The genome size ranges from approximately 27 to 34 kilobases. It
has a 5' methylated cap "5'leader, UTR", replicas/transcriptase enzyme, spike (S) protein,
envelope (E) protein, membrane (M) protein, nucleocapsid (N) protein, 3'untranslated region
(3'UTR), and a poly (A) tail, respectively. The S protein has a major role in eliciting the
protective immunity during infection with SARS-corona virus (SARS-CoV) by inducing
neutralizing-antibodies and T-cell responses. It is also the most mutated part of the
coronavirus genome. The aim of this effort is to study host-pathogen interaction in Egyptian
patients infected with COVID-19. The investigators will perform genome-wide miRNA and
transcriptome screens in the infected patients along with healthy ones for comparison. All
types of cytokines play pivotal roles in immunity, including the responses to different viral
infections. Therefore, The investigators will study the cytokines profile in response to that
infection. By comparing miRNA and transcriptome screens along with cytokines profiles, an
important molecule might be identified, which could play role in the inhibition of the
COVID-19 outbreak. In addition, this information will help us gaining awareness of the immune
process and knowing about the genes involved in the immune response against COVID-19 with an
emphasis on the expression of cytokines.
Genetic: COVID-19 Diagnostic and Assessment Tests
Complete Blood Count (CBC), Differential leukocytes, D-dimer, C-Reactive Protein, Polymerase Chain Reaction (PCR) Test, Ferritin level, Cytokines Profile, Transcriptome Analysis, miRNA Analysis.
Inclusion Criteria:
- Confirmed COVID-19 Patients, and patients aged above 18 will be considered in this
project.
Exclusion Criteria:
- Patients with more than one of these chronic diseases; diabetes mellitus,
hypertension, cardiac diseases, and livers diseases grade 2 and 3 will be excluded.
Tanta University Hospital
Tanta, El Gharbia, Egypt
Center of Excellence in Cancer Research, Tanta University Hospital.
Tanta, Gharbia, Egypt
Mohammed El Shanshoury, Professor
+201005680834
elshanshory@gmail.com
Mohammed Eid, Doctor, Principal Investigator
Botany and Microbiology Department, Faculty of Science, Tanta University Tanta