Hospitals are recognised to be a major risk for the spread of infections despite the availability of protective measures. Under normal circumstances, staff may acquire and transmit infections, but the health impact of within hospital infection is greatest in vulnerable patients. For the novel coronavirus that causes COVID-19, like recent outbreaks such as the SARS and Ebola virus, the risk of within hospital spread of infection presents an additional, significant health risk to healthcare workers. Infection Prevention and Control (IPC) teams within hospitals engage in practices that minimise the number of infections acquired within hospital. This includes surveillance of infection spread, and proactively leading on training to clinical and other hospital teams. There is now good evidence that genome sequencing of epidemic viruses such as that which causes COVID-19, together with standard IPC, more effectively reduces within hospital infection rates and may help identify the routes of transmission, than just existing IPC practice. It is proposed to evaluate the benefit of genome sequencing in this context, and whether rapid (24-48h) turnaround on the data to IPC teams has an impact on that level of benefit. The study team will ask participating NHS hospitals to collect IPC information as per usual practice for a short time to establish data for comparison. Where patients are confirmed to have a COVID-19 infection thought to have been transmitted within hospital, their samples will be sequenced with data fed back to hospital teams during the intervention phase. A final phase without the intervention may take place for additional information on standard IPC practice when the COVID-19 outbreak is at a low level nationwide.
Hospitals are recognised to be a major risk for the spread of infections despite the
availability of protective measures. Under normal circumstances, staff may acquire and
transmit infections, but the health impact of nosocomial infection is greatest in vulnerable
patients. For COVID-19, like SARS-CoV, MERS-CoV and Ebola virus, the risk of nosocomial
spread of infection presents an additional and significant health risk to healthcare workers
(HCW). During epidemics, normal infection prevention and control (IPC) practice is further
complicated by the difficulties of distinguishing community- and hospital-acquired
infections. This can lead to erroneous identification of nosocomial transmission, involving
unnecessary IPC efforts, while true nosocomial transmissions are missed thereby putting
patients and HCW at increased risk.
There is now good evidence that genome sequencing of epidemic viruses, together with standard
IPC, better excludes nosocomial transmissions and, depending on the virus, better identifies
routes of transmission, than IPC alone1-3. To date, all studies have been retrospective.
However, the development of rapid nanopore sequencing methods enables identification of
potentially linked or unlinked viruses within 24-48 hours: this timescale is short enough to
inform clinical IPC decisions in near-real-time. Although COVID-19 has a low mutation rate
estimated at around 2.5 changes per genome per month, it is generally agreed that sufficient
viral diversity now exists to identify where patient and staff infections that are apparently
clustered in time and space, are in fact due to different COVID-19 genotypes4. Such
information would rapidly exclude nosocomial transmission as the cause of the cluster, reduce
the need for IPC intervention and provide reassurance to healthcare workers that IPC measures
including personal protective equipment (PPE), had not been breached. However, confirmation
of COVID-19 transmission to patients and healthcare workers may be more difficult with a
single observed mutation between two genomes feasibly representing anything between one and
ten transmissions. Identical genomes will not necessarily provide evidence of a link between
two cases. Nonetheless by placing genotypes detected within the framework of all the
genotypes detected within the hospital setting, the surrounding community and COG-UK as a
whole, it may be possible to postulate nosocomial transmission where comparatively uncommon
genotypes are apparently linked or cluster in time and space.
The COG-UK initiative, which aims to sequence as many COVID-19 viruses as possible across the
UK thus provides an important and unique opportunity to test whether viral sequence data
produced in near-real-time, in addition to providing valuable information for public health
planning, could also reduce uncertainties around nosocomial transmission events, better
target IPC effort, improve hospital functioning and reduce the role of hospitals as a source
of infection to the community.
To address this,the investigators propose an adjunctive study, COG-UK HOCI. COG-UK HOCI will
take advantage of the COG-UK design, with its mixed model of smaller sequencing hubs located
close to hospitals and a large centralised hub sequencing most viruses, to identify not only
whether rapid viral sequencing is useful for patient management but how time-critical this
might be ; turnaround times for sequence data from the central hub are likely to be longer
(5-7 days) than those from local sequencing hubs (<48 hours).
COG-UK HOCI, by defining and reporting COVID-19 genotype frequencies within its participating
hospitals, as compared to those in the wider community, will also have the potential to
overcome some of the inherent barriers to identifying the likely sources of HOCI. The data
generated will provide as accurate as possible a picture, given the constraints of viral
genetic diversity, of numbers of COVID-19 infections being acquired by nosocomial
transmission and where these transmissions are occurring. While COG-UK will provide data on
the utility of viral genomics for national public health planning, COG-UK HOCI will quantify
the utility of the same data for local management of nosocomial infection, whether observed
benefits are time dependent and deliver the best estimates of how viral sequence data can be
used to quantify HOCI.
The outputs from COG-UK HOCI will further inform decisions about the likely future use of
viral genome sequencing for the management of epidemics and pandemics and how it might best
be organised, centralised or diversified, to deliver maximal impact.
The overarching aim of this study is to determine the utility of whole-genome sequencing to
provide additional insight into hospital-onset COVID-19 infections (HOCI) which, in turn, can
optimise IPC measures. In addition, the project aims to provide early data to help quantify
HOCI events and where these are occurring. These will contribute to local trust level
planning and to understanding of the role of HOCIs in contributing to COVID-19 outcomes and
spread.
Specifically, the study will determine the role of real-time availability of COVID-19
sequence data:
- In conjunction with routine IPC data, to identify and characterise HOCI
- To identify and characterise HOCI not previously identified by routine IPC data
- To generate estimated numbers of HOCI and where these are occurring
- To identify linked HOCI and hospital outbreaks
- To identify ways to reduce the incidence of HOCI
- In optimising IPC actions, e.g. by reducing the need for extra cleaning, ward closures
etc where a hospital outbreaks are excluded
- In changing workload, e.g. by reducing the need for extra cleaning, ward closures etc
where a hospital outbreaks are excluded Augmenting these approaches, the investigators
will measure whether the above are influenced by the time to sequence data result.
COG-UK HOCI is a phase III prospective, interventional, cohort, superiority study.
Allocation to either rapid local sequencing (c.24-48h) or lack of rapid local sequencing
(i.e. via Wellcome Sanger Institute at >96h) will be dependent on the time of the study (see
timelines).
Proposed study duration: 12 months; comprising 6 months of set-up, baseline data collection,
interventional data collection) and up to 6 months of data cleaning, data analysis and
reporting.
Other: Use of virus (Covid-19) genome sequence report to inform infection prevention control procedures
Rapid or standard (time to return to sites) receipt of virus (Covid-19) genomic sequencing reports
Inclusion Criteria:
- Participants must have confirmed COVID-19 infection and either:
1. be a potential hospital-onset COVID-19 infection (HOCI); or
2. potential workplace infection from COV-SARS-2 for site-based healthcare workers.
- Participants must have provided nasal swab/pharyngeal swab / combined nasal and
pharyngeal swab / nasopharyngeal aspirate or broncho alveolar lavage sample for
evaluation in the COG-UK project.
- Participants may be of any age to be included in study For clarity, in the above
criterion a potential HOCI is an admitted patient at site with first confirmed test
for COVID-19 >48 hours after admission, where they were not suspected to have COVID-19
at time of admission.
Exclusion Criteria:
- There are no exclusion criteria for COG-UK HOCI
University College London Hospitals NHS Foundation Trust
London, United Kingdom
Judith Breuer, MD, Principal Investigator
University College, London