The recent and unexpected occurrence of patients with the development of skin lesions onthe hands and/ or feet has been described recently. As these cases occurredcontemporaneously with the Coronavirus Disease 2019 (COVID-19) and as it was the mostoften occurrence of de novo frostbites, the question raised of whether there is a directlink between the occurrence of these lesions and infection of the severe acuterespiratory syndrome coronavirus 2 (SARS-CoV-2) the responsible for CoVID-19. Indeed,mechanisms of these lesions and the precise correlation with Sars-CoV-2 remains poorlyunderstood. Therefore, this study aim to: 1. Determine the possible link with this virus, 2. Understand the mechanisms involved in the pathogenesis of these lesions.
In this study, the investigator designed a prospective interventional study in order to
collect blood samples and skin lesion biopsy at significant time points in patients who
present a skin lesion. The intervention in this study is limited to some blood samples
and skin biopsies.
Two 7 mL tubes of blood will be collected at consultant time for essentially serological
test for the SARS-CoV-2 and for analysing certain populations of white blood cells. The
second additional blood sample of 7 mL will be collected around day 15 after inclusion in
a dry tube to perform a serological test for SARS-CoV-2 at a distance from the onset of
skin signs.
A biopsy for histological study is usually done in patients with acrosyndrome. This is
why this type of biopsy will be done as a routine care of these patients. An
immunohistochemical study could complete the histological explorations using an
anti-coronavirus antibody if arguments in favour of SARS-CoV-2 are demonstrated. In this
study, patients who accept and sign informed consent will be proposed a skin biopsy on a
characteristic lesion for freezing. This biopsy can be used for transcriptomic analysis.
This may allow a better understanding of the mechanisms of the disease.
Other: Blood sampling
Recruited patients will be subjected to 2 blood samples of 7 mL at day 0 for serological
analysis and cell collection and to an additional blood sample of 7 mL at day 15 for
serological analysis
Other: Skin biopsy
Recruited patients will be subjected to a single skin biopsy at day 0 for histological
and transcriptomic analysis
Inclusion Criteria:
- Adult patients
- Consultant for the recent occurrence of lesions on the fingers and or toes
- Having signed an informed consent
- Affiliated or entitled to a social security regime
Exclusion Criteria:
- Patients under guardianship or under curate
- Patients under state medical assistance
Department of Dermatology, Tarnier hospital, AP-HP
Paris, France
Investigator: Sélim Aractingi, MD, PhD
Contact: +33158411813
selim.aractingi@aphp.fr
Sélim Aractingi, MD, PhD
+33158411813
selim.aractingi@aphp.fr
Marie Benhammani-Godard
00.33158411190
marie.godard@aphp.fr