Assessment of Long-term Effects (Complications) of COVID-19 in the Southern Part of Bangladesh - a Retrospective Observational Study.

B. INTRODUCTION:

An outbreak of the novel coronavirus nCoV-19 (SARS-CoV-2), responsible for the coronavirus
disease-19 (COVID-19), was first detected in Hubei province, Wuhan, China, on December 31,
2019. It has rapidly spread globally with approximately 157,343,044 confirmed cases and
3,278,510 deaths till 7th May, 2021 [1]. World Health Organization (WHO) declared COVID- 19
pandemic on 11th March 2020.

The world is facing the second wave of Coronavirus Disease 2019 (COVID-19) pandemic which is
the most troublesome challenge to public health. The second wave is running and nobody knows
where we are in the course of this disease. It becomes a significant challenge for the public
health, science, and medical sectors [2].

According to the World Health Organization, about 80% of infections are mild or asymptomatic,
15% result in moderate to severe symptoms (requiring oxygen) and about 5% are critical
infections, which require ventilation.

We are learning something new every day. Our understanding of the pandemic is growing and
changing daily. The world is focusing on the short term - flattening the curve, treating the
sick and discovering a vaccine. But there is more to this pandemic than the short term.

We know a lot about the transmission and clinical feature of COVID-19, but relatively little
about what happens after someone recovers. Much is still unknown about how COVID-19 will
affect people over time. There's still much to be learned from those who have recovered from
COVID-19.

We are still only one year and four months beyond the first emergence of COVID-19, so it's
too early to tell what the extent of any long-term impacts will be.

Most people who have COVID-19 recover completely within a few weeks [3]. But some people -
even those who had mild disease, continue to experience symptoms after their initial
recovery. Older people and people with many serious medical conditions like diabetes, COPD,
heart disease, kidney disease etc. are the most likely to experience these lingering COVID-19
symptoms. The most common symptoms that linger over weeks, even months are:

- Fatigue

- Cough (dry)

- Shortness of breath

- Headache

- Joint pain

- Muscle pain

- Confusion and even hallucinations. But, our body has surprising healing capacity; most
of these symptoms will improve by six months. However, some people recovering from
COVID-19 will likely suffer from long-term health problems.

Although COVID-19 is seen as a disease that primarily affects the lungs, it can damage many
other organs as well. This organ damage may increase the risk of long-term health problems.
Some of the most common long-term effects are:

Lungs: The pneumonia associated with COVID-19 can often cause long-standing damage to the
tiny air sacs (alveoli) in the lungs [4]. The resulting scar tissue i.e. pulmonary fibrosis
can lead to long-term breathing problems.

Heart: A recent study from the University of Frankford in Germany showed abnormal heart
findings in more than 75% of people studied who had recently recovered from COVID-19. Imaging
tests taken months after recovery from COVID-19 have shown lasting damage to the heart
muscle, even in people who experienced only mild COVID-19 symptoms. This may increase the
risk of heart failure or other heart complications in the future.

Brain: COVID-19 can cause strokes [5], seizures and Guillain-Barre syndrome, even in young
people. COVID-19 may also increase the risk of developing Parkinson's disease and Alzheimer's
disease.

Blood clots and blood vessel problems: COVID-19 can predispose to form clots. While large
clots can cause heart attacks and strokes, much of the heart damage caused by COVID-19 is
believed to stem from very small clots that block tiny blood vessels (capillaries) in the
heart muscle. Other organs affected by blood clots include the lungs, legs, liver and
kidneys.

Psychiatric issues: Most people recovering from COVID-19 experience anxiety, depression, or
cognitive impairment. Some may develop more serious Post-Traumatic Stress Disorder (PTSD) due
to the trauma of the illness and treatment [6].

Kidney: Patient may develop kidney complications which could lead to a need for long-term
dialysis.

A lot of things may happen in long run in those who recovered from COVID-19. Our
understanding of COVID-19's long term effects will depend greatly on ongoing studies over the
next decades.

C. OBJECTIVES:

1. General Objectives:

To assess long-term effects of COVID-19 in patients who were affected with COVID -19
(RT-PCR +ve) from 1st July to 30th December, 2020 recorded in PCR Lab of Sher-E-Bangla
Medical College Hospital, Barishal i.e. six months or more before the initiation of
study and recovered fully.

2. Specific Objective:

To detect the system-based complications and assess their incidence, and compare the results
with other studies in home and abroad.

D. METHODOLOGY:

Study Design: An observational retrospective study. Study Population: COVID -19 (RT-PCR +ve)
patients from 1st July to 30th December, 2020 recorded in PCR Lab of Sher-E-Bangla Medical
College Hospital, Barishal.

Inclusion Criteria:

- COVID-19 (RT-PCR +ve) patients age 20 years and above.

- Those patients whose mobile numbers are recorded in the register.

- Those patients who/their attendants are available on mobile phone.

Exclusion Criteria:

- Persons below 20 years of age.

- Those patients whose mobile numbers are not recorded in the register.

- Those patients who/their attendants are not available on mobile phone.

- Those who died by this time from causes unrelated to COVID-19.

Sample Size (n):

To determine the sample size the following formula was followed n= n= the desired sample size
z= Standard normal deviate usually set at 1.96 p= Proportion in the population; if not known,
it is regarded as 0.5 (50.0%), here p is 0.27 q= 1-p d= Degree of accuracy which is
considered as 0.05 According to this formula the targeted sample was 302. But attempt will be
taken to take more sample population as far as possible.

Sampling Technique:

Simple random sampling.

Place of Study:

Sher-E-Bangla Medical College Barishal Bangladesh.

Time-frame & Duration:

6 months from 1st July to 30th December 2021.

Research Instrument:

1. Record register of PCR lab

2. Printed questionnaire

3. Mobile phone

4. Prescription of registered doctor if any

5. Investigations if any.

Case definition:

1. Long-term effects of COVID-19 are those complications which develop or persist after 6
(six) months of COVID-19.

2. System-based complications should follow the standard definition and are listed in the
questionnaire.

Statistical Analysis:

Statistical analyses will be carried out by using the Statistical Package for Social Sciences
version 23 for Windows (SPSS Inc., Chicago, Illinois, USA). The mean and Standard deviation
values will be calculated for continuous variables. t- test and analysis of variance (ANOVA)
will be employed for continuous data. P values <0.05 was considered as statistically
significant.

E. PROCEDURE:

The proposed study will be conducted in Sher-E-Bangla Medical College, Barishal, Bangladesh.
Those who were RT-PCR positive for corona in PCR Lab of Sher-E-Bangla Medical College,
Barishal from 1st July to 30th December, 2020 and recovered fully, will be listed from
register of PCR Lab and their contact numbers will be collected from there. They will be
contacted via cell phone and information will be collected after initial exchange of
wellbeing and verbal consent. The conversation will be conducted by a registered physician as
per prescribed questionnaire. If any complication is reported, then that will be confirmed
either by the diagnosis of a registered physician (if already seen) or the patient will be
advised to come and confirmed by respective specialist according to complication and specific
investigation (if available). Follow-up will be done for at least 6 months after 6 months
from the date of RT-PCR +ve by 3 telphonic conversation. All information will be registered
in data collection form and analysed thereafter.

F. QUALITY CONTROL:

The quality control of this study will be supervised under the guidance of Professor Khan AK
Azad, Ex. Principal, Professor and Head of the Department of Medicine, Dhaka Medical College,
Dhaka under the SOP (Standard Operative Procedure).

G. INSTITUTIOAL APPROVAL:

We have already taken the institutional clearance from chairman, ethical committee,
Sher-E-Bangla Medical College, Barishal, Bangladesh.

H. BUDGET:

Total Budget: BDT 3,50,000 (Approximately).

Detailed of the Budget:

Particulars BDT Doctor's salary- 1x6 months x 20000 120,000/- Data entry computer personel
(Research associate's monthly salary 10000/-X 6) 60,000/= Specialist visit (Approximate)
40,000/= Investigations (Approximate) 1,00000/- Statistical analysis 15,000/= Stationary cost
10,000/= Others 5,000/= Total in Amount 3,50,000/=

I. FUNDING:

Contribution from researchers

J. OUTCOME:

At the end of study, we will be able to assess long-term effects of COVID-19 in patients of
southern part of Bangladesh and to know its incidence. It will be possible to compare this
with other studies in Bangladesh and other countries.

K. CONFLICTS OF INTEREST:

The investigator will not have any salary for himself from the fund. The salary will be
provided to doctor, research associates and statistician.