There is little information on the characteristics of airborne severe acute respiratory syndrome coronavirus containing aerosols, their concentration, or their infectivity.The aim was to determine airborne severe acute respiratory syndrome coronavirus transmission, their infectivity in different areas such as patient's room and in medical staff área.
The Corona Virus Disease 2019 is rapidly spreading throughout the world. Aerosol is a
potential transmission route.
The transmission of severe acute respiratory syndrome coronavirus in humans is thought to be
via at least 3 sources: 1) inhalation of liquid droplets produced by and/or 2) close contact
with infected persons and 3) contact with surfaces contaminated with severe acute respiratory
syndrome coronavirus. There are many respiratory diseases spread by the airborne route such
as tuberculosis, measles and chickenpox. Several studies suggested that airborne spread may
have played an important role in the transmission of that disease. At present, there is
little information on the characteristics of airborne severe acute respiratory syndrome
coronavirus containing aerosols, their concentration, or their infectivity.
The aim was to determine airborne severe acute respiratory syndrome coronavirus 2
transmission, their infectivity in in different areas such as patient's room and in medical
staff area.
Methods
Cross sectional study of samples from environmental of patient´s room, and medical staff
area. Samples will be taken of air using microbial air monitoring systems, and from different
surfaces of patient's room and medical staff area, such as computers, mouse and personal
protective equipment using cotton swabs. Viral RNA will be determined using
Real-Time-Polymerase-Chain Reaction. In positive samples infectiveness will be determined by
cell culture under biosecurity conditions.
Statistical analysis will be performed to determine prevalence of positive samples and
positive cell culture whit STATA version 15.1.
informed consent will be obtained from all participants
Other: Severe Acute Respiratory Syndrome CoronaVirus 2 detection
Severe Acute Respiratory Syndrome CoronaVirus 2 Detection by Real-Time Polymerase Chain Reaction and cell culture
Inclusion Criteria:
- Adults 18 years and more, with diagnoses of COVID-19 96 hours since the beginning of
symptoms.
- Medical staff, nurses and/or physician taking care of patient whit COVID-19
Exclusion Criteria:
- Unwilling to give informed consent
Hospital Italiano de Buenos Aires
Ciudad autónoma de Buenos Aires, Argentina